RNAi Mediated Transgenesis for Improving Animal Produce

RNAi mediated gene knockdown and transgenesis by microinjection in the necromenic Nematode Pristionchus pacificus.

Although it is increasingly affordable for emerging model organisms to obtain completely sequenced genomes, further in-depth gene function and expression analyses by RNA interference and stable transgenesis remain limited in many species due to the particular anatomy and molecular cellular biology of the organism. For example, outside of the crown group Caenorhabditis that includes Caenorhabdit...

Effective RNAi-mediated β2-microglobulin loss of function by transgenesis in Xenopus laevis

To impair MHC class I (class I) function in vivo in the amphibian Xenopus, we developed an effective reverse genetic loss of function approach by combining I-SceI meganuclease-mediated transgenesis with RNAi technology. We generated transgenic outbred X. laevis and isogenetic laevis/gilli cloned lines with stably silenced expression of β2-microglobulin (b2m) critical for class I function. Trans...

Spermatogonial Stem Cells and Animal Transgenesis

Relations between animal transgenesis and reproduction.

Transgenesis has become an essential tool for the study of gene expression mechanisms and functions. Transgenesis is also more and more used for biotechnological applications such as the study of human diseases, the adaptation of pig organs to humans, the production of pharmaceutical proteins in milk and likely in the future for the improvement of animal production. The use of transgenesis reli...

Transgenesis in Animal Agriculture: Addressing Animal Health and Welfare Concerns

The US Food and Drug Administration’s final Guidance for Industry on the regulation of transgenesis in animal agriculture has paved the way for the commercialization of genetically engineered (GE) farm animals. The productionrelated diseases associated with extant breeding technologies are reviewed, as well as the predictable welfare consequences of continued emphasis on prolificacy at the pote...